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目的了解宁夏地区汉族人群白细胞介素-6基因启动子区域634C/G位点单核苷酸多态性与汉族煤工尘肺的相关性。方法应用聚合酶反应-限制性片段长度多态性法检测120名健康接尘体检者和120例CWP患者的IL-6(-634C/G)位点的基因型,比较各基因型在2组间检出率的差别。结果煤工尘肺组与煤尘接触组在接尘工龄、工种构成、吸烟烟龄差异无统计学意义(P﹥0.05)。对年龄进行标化后,分析发现基因型CC和CG+GG在宁夏地区煤工尘肺组和煤尘接触组分布频率分别为66.7%、33.3%和52.5%、47.5%,两组比较差异有统计学意义(P﹤0.05);等位基因C在两组分布差异有统计学意义(P﹤0.05)。结论该研究发现IL-6(-634C/G)位点多态性与宁夏地区煤工尘肺病的发生可能存在相关性。
Objective To understand the association between single nucleotide polymorphism at 634C / G locus of interleukin-6 gene promoter and pneumoconiosis in Han nationality in Ningxia. Methods The genotypes of IL-6 (-634C / G) in 120 healthy controls and 120 CWP patients were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotypes of IL-6 The difference between the detection rate. Results Coal dust pneumoconiosis group and coal dust exposure group had no significant difference in the service life of dust, the type of work and the smoking age (P> 0.05). After the standardization of age, the frequencies of genotypes CC and CG + GG were found to be 66.7%, 33.3%, 52.5% and 47.5% respectively in coal miners’ pneumoconiosis and coal dust exposure groups in Ningxia. There were statistically significant differences between the two groups (P <0.05). There was significant difference in allele C distribution between the two groups (P <0.05). Conclusion The study found that IL-6 (-634C / G) locus polymorphism may be related to the occurrence of coal worker’s pneumoconiosis in Ningxia.