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１９９０年后，云南省逐步开展了脊髓灰质炎（脊灰）病毒学监测，１９９９０～１９９７年共分离到“株脊灰病毒．对毒株进行了型内鉴定及核苷酸序列分析，并及时根据野毒监测结果开展了针对性的免疫活动。７年的监测及应用研究结果表明：（１）１９９０～１９９３年，云南省有脊灰Ⅰ型野病毒的广泛传播和循环；（２）１９９３年后所采取的常规免疫辅以强化免疫的干预措施具有明显的控制效果；（３）在我国首次监测到输人性脊灰野病毒病例．确定了云南省边境地区和周边地区已成为脊灰高危地区，是世界卫生组织西太平洋地区和中国今后工作的重点和难点；（４）必须加强边境地区的免疫和监测工作，同时还应加强地区间、国家间和大区间的合作与协调，才能确保全球消灭脊灰目标的实现．
After 1990, the poliovirus surveillance of polio (polio) was gradually carried out in Yunnan Province, and strains of poliovirus were isolated from 19990 to 1997. The strains were identified by in-type and nucleotide sequence analysis, and in time According to the results of wild virus surveillance, targeted immunization activities were carried out.The results of seven years of monitoring and applied research showed that: (1) The widespread transmission and circulation of wild-type wild type I wild virus in Yunnan Province from 1990 to 1993; (2) 1993 Year after the routine immunization combined with intensive immunization intervention has obvious control effect; (3) in our country for the first time to detect cases of human transmission of wild poliovirus.Determine the border area of Yunnan Province and the surrounding areas have become polio high-risk areas Is the focus and difficulty of WHO’s work in the Western Pacific Region and China in the future; (4) The need to strengthen immunization and surveillance in border areas and strengthen cooperation and coordination among regions, countries and regions should ensure that the global Achieve the goal of eliminating polio.