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为利用白僵菌防治桑天牛,从野外感病死亡昆虫体上进行分离,同时利用黄粉虫对河北省土壤中的白僵菌进行诱集。诱集结果表明:白僵菌较多存在于自然的或人为活动较少的土壤中。利用获得的菌株对桑天牛幼虫进行生物测定,初步筛选出Bi05和Bs042个致病力较强的菌株,其接种10d后的死亡率分别为96.47%和92.93%,致死中时分别为4.53d和4.83d,而其他菌株的死亡率在28.59%和82.12%之间,致死中时从5.84d到16.4d。利用不同孢子浓度的悬浮液对桑天牛幼虫进行接种,进一步测定了Bi05和Bs04菌株的致死中浓度分别为6.76×105和1.39×106conidia·mL-1。Bi05和Bs04菌株具有生物防治桑天牛的潜力。
In order to utilize Beauveria bassiana to control Morinda Cows, we isolated it from wild insects and used Bentley bug to trap Beauveria bassiana in the soil of Hebei Province. Trapping results show that: Beauveria bassiana is more in natural or less human activity in the soil. The bioassay of S. mori larvae was carried out by using the obtained strains, and the strains with strong pathogenicity of Bi05 and Bs042 were screened out initially. The mortality rates after 10 days of inoculation were 96.47% and 92.93%, respectively, and were 4.53 days And 4.83d respectively, while the mortality of other strains was between 28.59% and 82.12%, and from 5.84d to 16.4d when they were dead. Inoculation of mulberry larvae with different concentrations of spore suspension further determined that the lethal concentrations of Bi05 and Bs04 strains were 6.76 × 105 and 1.39 × 106conidia · mL-1, respectively. Bi05 and Bs04 strains have the potential of biological control of Morus alba.