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探讨大肠腺癌DNA和RNA含量的变化意义及预后的关系。方法 :对我院 1985年～ 1992年收治的术后随访 74例病人的大肠腺癌石腊标本重新切片 ,用流式细胞技术进行DNA和RNA含量测定 ,探讨其变化意义与转移预后的关系。结果 :大肠腺癌DNA异倍体与组织学类型无关 ,RNA含量与组织学类型关系密切。DNA异倍体和RNA含量大肠腺癌的Dukes’分期、淋巴结转移、其它脏器转移和根治术后复发均有关。术后生存 5年以上病人的DNA异倍体出现率和RNA含量较术后 1年内死亡病人为低 ,两者有显著性差异 (P <0 .0 1)。结论 :DNA和RNA含量测定可作为判断大肠腺癌的恶性程度、估价预后的客观可靠指标。
To investigate the relationship between the changes of DNA and RNA in colorectal adenocarcinoma and its prognosis. Methods: A total of 74 patients with colorectal adenocarcinoma were paraffin sectioned from 1985 to 1992 in our hospital. The DNA and RNA contents were determined by flow cytometry to explore the relationship between the changes and the prognosis of metastasis. Results: DNA aneuploidy in colorectal adenocarcinoma was not related to the histological type. The content of RNA was closely related to histological type. DNA aneuploidy and RNA content of Dukes’ stage colon adenocarcinoma, lymph node metastasis, other organ metastases and recurrence after radical surgery are related. The frequency of DNA aneuploidy and the RNA content in patients who survived more than 5 years after operation were lower than those who died within 1 year after operation, with significant difference (P <0.01). Conclusion: The determination of DNA and RNA levels can be used as an objective and reliable indicator of the prognosis of colorectal adenocarcinoma.