黄连解毒汤对阿尔茨海默病模型斑马鱼认知功能障碍的影响

来源 :中国药理学与毒理学杂志 | 被引量 : 0次 | 上传用户:clearshine
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目的探究黄连解毒汤对阿尔茨默病(AD)斑马鱼认知功能障碍的影响。方法将126 g黄连,84 g黄柏,84 g黄芩,126 g栀子分别放入10,8和8倍量的水,浸泡30 min,然后100℃分别提取2,1.5和1.5 h,将3次提取液粗滤浓缩为308 g·L-1放入冰箱备用。选取200尾斑马鱼于第1~7天在T型缸进行行为学训练,结束后录像并利用行为学记录分析软件smart3.0进行行为学分析,选出合格斑马鱼,为空白组。除空白组斑马鱼不做任何处理外,其余5组于第8~13天暴露在AlCl3
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目的阿尔茨海默病(AD)是世界第一的神经退行性疾病,目前缺乏有效的治疗药物。研究发现,AD与2型糖尿病(T2DM)之间存在密切联系。课题组系统分析了19种糖尿病治疗复方,从中筛选出具有AD治疗作用的黄芪葛根汤,并通过网络药理学以及现代药理学基础分析探讨黄芪葛根汤治疗AD的潜在靶点及信号通路,阐述其可能的作用机制。方法采用Aβ1-42基因诱导的秀丽隐杆线虫作为AD病理模型,探讨黄芪葛根汤的抗AD作用。从TCMSP和BATMAN-TCM数据库搜索黄芪葛根汤中2味中药的化合物及靶点,使用GeneCards、NC
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