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目的:观察缓释载药体泊洛沙姆407凝胶在听泡内外的降解过程,探讨其在内耳疾病局部缓释治疗中的应用前景。方法:定时测量37℃条件下泊洛沙姆407凝胶的降解量;并于健康豚鼠右侧听泡内灌注100μl20%泊洛沙姆407溶液作为实验组。术后7d、14d、28d及49d行听泡石蜡包埋连续切片,分别观察凝胶在听泡内的生物降解情况及凝胶对中耳腔黏膜、圆窗膜的影响。结果:20%和25%泊洛沙姆407凝胶各1ml在37℃条件下49d可分别降解(78.89±13.10)μg和(75.32±8.94)μg;14d时听泡内少量凝胶残留;光镜下49d时残留少量絮状凝胶物。结论:泊洛沙姆407凝胶体外降解较慢,听泡内可以生物降解和经咽鼓管排出两种形式减少,对听泡组织无致炎及排斥作用,适合于做内耳病短期缓释药物治疗的载体。
OBJECTIVE: To observe the degradation process of sustained-release drug-loaded poloxamer 407 gel inside and outside of the auditory bulb, and to explore its application prospect in local slow-release treatment of inner ear diseases. Methods: The degradation of poloxamer 407 gel at 37 ℃ was measured regularly. 100 μl of 20% poloxamer 407 solution was infused into the right side of healthy guinea pigs as experimental group. After 7 days, 14 days, 28 days and 49 days, the paraffin-embedded serial sections were observed. The biodegradation of the gel in the auditory bulb and the effect of gel on the mucosa and round window of the middle ear were observed. Results: 1 ml of 20% and 25% poloxamer 407 gels degraded (78.89 ± 13.10) μg and (75.32 ± 8.94) μg, respectively, at 49 ℃ for 49 days. Microscopic 49d left a small amount of flocculent gel. CONCLUSION: Poloxamer 407 gel degrades slowly in vitro and can be biodegraded within the auditory bulb and eustachian tube discharge, reducing the inflammation and exclusion of the auditory bud tissue. It is suitable for short-term sustained release of inner ear disease Medical treatment of the carrier.