论文部分内容阅读
目的观察色素上皮衍生因子(PEDF)、晚期糖基化终产物受体(RAGE)的表达及重组PEDF对RAGE表达的抑制作用,探讨PEDF与DN的关系及对DN的保护作用。方法采用糖化小牛血清白蛋白(AGE-BSA)体外诱导人肾小球系膜细胞(HRMCs),Western blot及RT-PCR法分别检测RAGE、PEDF蛋白和mRNA表达。结果 (1)AGE-BSA(100~400mg/L)呈浓度梯度减少HRMCs PEDF表达(P<0.01),升高RAGE表达(P<0.01);(2)重组PEDF蛋白(5~40nmol/L)呈浓度依赖性抑制AGE-BSA介导RAGE蛋白在HRMCs的表达(P<0.05)。结论 AGEs通过降低PEDF表达,增加RAGE表达参与DN的发生,PEDF可能通过抑制AGE-RAGE轴对DN发挥保护作用。
Objective To observe the expression of PEDF, RAGE and the inhibitory effect of recombinant PEDF on RAGE expression, and to explore the relationship between PEDF and DN and the protective effect on DN. Methods Human mesangial cells (HRMCs) were induced by using glycated bovine serum albumin (AGE-BSA) in vitro. The protein and mRNA expressions of RAGE and PEDF were detected by Western blot and RT-PCR respectively. Results: (1) The concentration gradient of AGE-BSA (100-400mg / L) decreased the PEDF expression in HRMCs (P <0.01) and increased the expression of RAGE (P <0.01) AGE-BSA inhibited the expression of RAGE protein in HRMCs in a concentration-dependent manner (P <0.05). Conclusion AGEs can reduce the expression of PEDF and increase the expression of RAGE in DN. PEDF may play a protective role on DN by inhibiting the AGE-RAGE axis.