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目的 为了探索一氧化氮(NO)、脂质过氧化物(丙二醛:MDA)和抗氧化物质(超氧化物岐化酶:SOD)在类风湿性关节炎(RA)发病机理中的作用。本文观察了正常人和PA患者血清及滑液中NO、SOD、MDA含量的变化。方法 采取70名健康成人血清和85名患者血清及滑液,NO含量用硝酸还原法测定,SOD活性用化学比色法测定,MDA含量用硫代巴比妥酸法测定。结果 (1)RA患者的血清NO含量高于正常对照组(F =2 5 0 5 ,P <0 0 1)。(2 )RA患者的血清SOD量低于正常对照组(F =2 0 5 7,P <0 0 1)。(3)RA患者的血清MDA量高于正常对照组(F =2 0 6 1,P <0 0 1)。(4)血清中SOD与NO、MDA呈明显负相关(血清NO :R =- 0 312 ,P <0 0 5。血清MDA :R =- 0 35 6 ,P <0 0 1)。结论 RA患者血清SOD活性下降、NO和MDA含量上升,表明RA发病早期体内自由基产生过多,使抗氧化物质的抗氧化活性下降,检测其活性有助于RA临床早期诊断。
Objective To explore the role of nitric oxide (NO), lipid peroxides (MDA) and antioxidants (superoxide dismutase SOD) in the pathogenesis of rheumatoid arthritis (RA) . This article observed the normal and PA patients serum and synovial fluid NO, SOD, MDA content changes. Methods Serum and synovial fluid of 70 healthy adults and 85 patients were collected. The content of NO was determined by nitrate reduction method. The activity of SOD was measured by chemical colorimetry. The content of MDA was measured by thiobarbituric acid method. Results (1) Serum NO levels in RA patients were higher than those in normal controls (F = 2550, P <0.01). (2) The serum level of SOD in patients with RA was lower than that of the normal control group (F = 2057, P <0.01). (3) The serum levels of MDA in RA patients were higher than those in normal controls (F = 2061, P <0.01). (4) Serum SOD was negatively correlated with NO and MDA (Serum NO: R = - 0 312, P <0 0 5. Serum MDA: R = -0 356, P <0.01). Conclusion Serum SOD activity is decreased and NO and MDA contents are increased in patients with RA. It indicates that excessive production of free radicals in early stage of RA leads to the decrease of antioxidant activity of antioxidants. Detecting its activity is helpful for early clinical diagnosis of RA.