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目的对显脉羊蹄甲中具有抑制血小板聚集活性的有效部位的化学成分进行研究。方法从显脉羊蹄甲抑制血小板聚集活性部位中,利用溶剂分步萃取法和各种色谱手段(硅胶吸附柱色谱,聚酰胺柱色谱,Sephadex LH-20柱色谱,开放ODS柱色谱,液相色谱等)分离得到了10个化合物,根据其理化性质和光谱数据分析(1H-NMR、13C-NMR等)鉴定了它们的结构。结果从活性部位中分离鉴定的化合物为黄酮类,分别是:3,5,7,4’-四羟基-3’-甲氧基黄酮(3,5,7,4’-tetrahydroxy-3’-methoxyflavone,1)、5,7,3’,4’-四羟基-3-甲氧基黄酮(5,7,3’,4’-tetrahydroxy-3-methoxyflavone,2)、槲皮素(quercetin,3)、木樨草素(luteolin,4)、山柰酚(kaempferol,5)、5,7,4’-三羟基-3’-甲氧基黄酮(5,7,4’-trihydroxy-3’-methoxyflavone,6)、7,3’,4’-三羟基-3-甲氧基黄酮(7,3’,4’-trihydroxy-3-methoxyflavone,7)、杨梅苷(myricitrin,8)、槲皮素-3-O-α-L-吡喃阿拉伯糖苷(quercetin-3-O-α-Larabinopyranoside,9)、儿茶素(catechin,10)。结论化合物1-6、8-9均为首次从该植物中分离得到,化合物7为首次从羊蹄甲属植物中分离得到。
Objective To study the chemical constituents of effective fractions with inhibitory activity on platelet aggregation in A. mirifica. Methods The active sites of platelet aggregation inhibited from the scutellaria officinalis, using solvent stepwise extraction and various chromatographic methods (silica gel adsorption column chromatography, polyamide column chromatography, Sephadex LH-20 column chromatography, open ODS column chromatography, liquid chromatography Etc.) 10 compounds were isolated and their structures were identified on the basis of their physicochemical properties and spectral data analysis (1H-NMR, 13C-NMR, etc.). Results The compounds isolated and identified from the active site were flavonoids: 3,5,7,4’-tetrahydroxy-3’-methoxyflavone (3,5,7,4’-tetrahydroxy-3’- Methoxyflavone, 1), 5,7,3’,4’-tetrahydroxy-3-methoxyflavone (5,7,3’,4’-tetrahydroxy-3-methoxyflavone,2), quercetin, 3), luteolin (4), kaempferol (5), 5,7,4’-trihydroxy-3’-methoxyflavone (5,7,4’-trihydroxy-3’ -methoxyflavone, 6), 7,3’,4’-trihydroxy-3-methoxyflavone (7), myricitrin (8), quinone Petritin-3-O-α-L-arabinofuranoside (9), catechin (10). Conclusion Compounds 1-6 and 8-9 were isolated from this plant for the first time, and compound 7 was isolated from the plant of the genus.