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用聚乙烯吡咯烷酮（PVP）预处理甘蔗顶端外植体，并以PVP作为附加成分直接加入培养基，均可取得防止接种物褐变坏死的良好效果；但同时发现，当加入培养基中的PVP达到一定浓度，则会表现出延缓分化芽增殖速度的不良作用；对PVP在植物组织培养中的应用作出评价．
Pretreatment of apical explants of sugarcane with polyvinylpyrrolidone (PVP) and direct addition of PVP as an additional component to the medium gave good results in preventing browning necrosis of the inoculum; however, it was also found that when PVP was added to the medium To reach a certain concentration, it will show the proliferation of delayed proliferation of the adverse effects; PVP plant tissue culture in the evaluation.