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在对乌骨鸡多肽的蛋白质含量、水解度及多肽的分子量分布研究基础上,通过测定乌骨鸡活性肽对邻苯三酚自氧化产生的超氧阴离子、Fenton反应产生的羟自由基、DPPH自由基的清除率,还原能力及抑制脂质体过氧化,全面考察了乌骨鸡多肽的体外抗氧化能力。结果表明:乌骨鸡多肽能明显降低邻苯三酚自氧化速率,对羟自由基有极强的清除能力,对DPPH自由基清除率随浓度增加而增加,其IC50分别为1.53、0.051.7mg/mL和1.60mg/mL;进一步研究表明,乌骨鸡多肽具有一定的还原力且与浓度呈正比例关系,同时也能抑制脂质体的过氧化反应。
Based on the research on the protein content, degree of hydrolysis and the molecular weight distribution of the polypeptide, the effect of the silky fowl active peptide on the superoxide anion generated by the autoxidation of pyrogallol, the hydroxyl radical generated by the Fenton reaction, the DPPH Free radical scavenging ability, reducing ability and inhibition of liposome peroxidation, a comprehensive study of Silky peptide antioxidant capacity in vitro. The results showed that the black-bone chicken peptide could significantly reduce the autoxidation rate of pyrogallol and had a strong ability of scavenging hydroxyl radicals. The DPPH radical scavenging rate increased with the increase of its concentration, with IC50 of 1.53 and 0.051.7 mg / mL and 1.60mg / mL; further studies have shown that silky polypeptide has a certain reducing power and the concentration was proportional to the relationship, but also inhibit liposome peroxidation.