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丝裂霉素Ｃ（ＭＭＣ）给药分为０．２５、０．５、１０和２．０ｍｇ／ｋｇ四个剂量组，同时设高生理盐水对照组。采用腹腔注射给药，２４ｈ取材。用吖啶橙荧光染色和Ｇｉｅｍｓａ染色方法研究了上海种、ＬＡＣＡ和Ｃ＿（５７）小鼠骨髓多染红细胞的微核率。结果表明，ＭＭＣ能诱发三种品系小鼠微核升高，敏感性最高的为上海种小鼠，与ＬＡＣＡ和Ｃ＿（５７）小鼠相比有非常显著性差异（Ｐ＜０．０１）。ＬＡＣＡ与Ｃ＿（５７）小鼠之间相比无明显的统计学差异。上海种小鼠腹腔注射ＭＭＣ２．０ｍｇ／ｋｇ后观察１２、２４、３６、４８和７２ｈ发现ＭＭＣ的作用高峰为２４ｈ。比较两种染色方法的小鼠微核率无明显差异。
Mitomycin C (MMC) was divided into four dose groups of 0.25, 0.5, 10 and 2.0 mg / kg, and high saline control group. Using intraperitoneal injection, 24h drawing. The micronuclei of erythrocytes in bone marrow of Shanghai, LACA and C57 mice were studied by acridine orange fluorescence staining and Giemsa staining. The results showed that MMC could induce the increase of micronuclei in three strains of mice, the highest sensitivity was Shanghai mice, which was significantly different from that of LACA and C57 mice (P <0.01). There was no significant difference between LACA and C57 mice. Shanghai mice intraperitoneal injection of MMC2.0mg / kg observed after 12, 24,36,48 and 72h found that the peak of MMC for 24h. There was no significant difference in micronucleus rate between the two staining methods.