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Knee osteoarthritis (KOA) is a chronic, progressive and cumulative disease, which pertains to the domain of rheumatism involving the bones and joints.The paroxysm is featured by alternation of acute and chronic pain.The effectiveness of the treatment with combination of acupuncture and Tuina has already been approved.However, studies on treatment with acupuncture and tissue separating manipulation were still quite limited.The author of the article has treated totally 70 cases of KOA with the combination of acupuncture and tissue separating manipulation in the recent 10 years, and the experience is now sharing as the follows.