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目的:探讨复方苦参汤对溃疡性结肠炎(UC)大鼠的作用及机制。方法:采用2,4,6-三硝基苯磺酸(TNBS)诱导溃疡性结肠炎大鼠模型,用不同剂量的复方苦参汤进行干预,检测结肠组织丙二醛(MDA)、一氧化氮合酶(i NOS)和一氧化氮(NO)水平,以及髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,观察UC大鼠一般情况和结肠组织形态变化。结果:UC大鼠结肠组织MDA(P<0.05)、i NOS(P<0.01)和NO(P<0.01)水平及MPO(P<0.01)活性显著高于正常对照组,SOD(P<0.01)和GSH-Px(P<0.05)活性显著低于正常对照组;复方苦参汤高剂量组治疗后可明显降低UC大鼠结肠组织中MPO(P<0.01)、MDA(P<0.05)、i NOS(P<0.05)和NO(P<0.01)水平,增加SOD(P<0.01)和GSH-Px(P<0.05)活性,其疗效呈剂量依赖性。同时大鼠的一般情况及结肠组织形态亦得到明显改善。结论:复方苦参汤能显著抑制UC大鼠氧化应激反应,减轻结肠炎症损伤,具有较高的临床治疗价值。
Objective: To investigate the effect and mechanism of Compound Kushen Decoction on ulcerative colitis (UC) rats. Methods: The rat model of ulcerative colitis was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS), and the compound Kushen Decoction was used to intervene to detect the malondialdehyde (MDA), monoxide Nitric oxide synthase (iNOS) and nitric oxide (NO) levels, as well as myeloperoxidase (MPO), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) The general situation of UC rats and the morphological changes of colon were observed. Results: Compared with normal control group, the activity of MDA (P <0.05), iNOS (P <0.01), NO (P <0.01) and MPO (P <0.01) (P <0.01), MDA (P <0.05) and GSH-Px (P <0.05) significantly lower than that of the normal control group NOS (P <0.05) and NO (P <0.01), and increased the activity of SOD (P <0.01) and GSH-Px (P <0.05) in a dose-dependent manner. At the same time the general situation of rats and colon morphology has also been significantly improved. Conclusion: Compound Kushen Decoction can significantly inhibit the oxidative stress in UC rats, reduce colonic inflammatory injury, and has high clinical value.