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目的:探讨转化生长因子(TGF－β)对口腔粘膜角化细胞增殖分化的调控作用及其机制。方法:对人口腔粘膜上皮角化细胞(KC)进行分离,传代培养和鉴定,加入含有不同质量浓度的 TGF－β培养液培养3 d,然后用四唑盐(MTT)比色试验检测TGF－β对 KC增殖状况的影响。结果:体外培养 KC呈多角形,胞浆内有丰富的张力细丝,表面有大量微绒毛,角蛋白抗体免疫组化呈阳性。TGF－β在一定的浓度范围内对KC的增殖起抑制作用。结论:TGF－β对体外培养的KC增殖有抑制作用。
Objective: To investigate the regulatory effect of transforming growth factor-β (TGF-β) on the proliferation and differentiation of oral mucosal keratinocytes and its mechanism. Methods: Human keratinocytes (KCs) were isolated, subcultured and identified. Culture medium containing different concentrations of TGF-β was cultured for 3 days. Then MTT colorimetric assay was used to detect the expression of TGF- β on the proliferation of KC. Results: KC was polygonal in vitro with abundant filament in cytoplasm, abundant microvilli on the surface and positive immunohistochemistry of keratin antibody. TGF-β inhibits the proliferation of KC in a certain concentration range. Conclusion: TGF-β can inhibit the proliferation of KC cultured in vitro.