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目的:建立反相高效液相色谱法同时测定厚朴温中滴丸中木香内酯、去氢木香内酯、6-姜酚、和厚朴酚与厚朴酚的含量。方法:采用ZORBAX SB-C18色谱柱(250mm×4.6mm,5μm),以甲醇-水(60∶40)为流动相,流速1.0mL.min-1,检测波长250nm,柱温30℃。结果:5个成分均达到基线分离,木香内酯、去氢木香内酯、6-姜酚、和厚朴酚、厚朴酚的线性范围分别为0.0633～0.633μg(r=0.9998),0.0822～0.822μg(r=0.9997),0.0701～0.701μg(r=0.9998),0.0461～0.461μg(r=0.9997),0.0482～0.482μg(r=0.9998);平均回收率(n=6)分别为97.62%,97.81%,97.16%,97.32%,96.55%。结论:本方法简单、可靠、专属性强,可以作为控制厚朴温中滴丸质量的方法。
Objective: To establish a reversed phase high performance liquid chromatography (HPLC) method for the simultaneous determination of ligustilide, dehydrocostus, 6-gingerol, honokiol and magnolol in Magnolia Bunge. METHODS: ZORBAX SB-C18 column (250 mm × 4.6 mm, 5 μm) was used with mobile phase of methanol-water (60:40) as mobile phase at a flow rate of 1.0 mL · min-1. The detection wavelength was 250 nm and the column temperature was 30 ℃. Results: The five components all reached baseline separation. The linear ranges of lignansolone, dehydrolactone, 6-gingerol, honokiol and honokiol were 0.0633 ~ 0.633μg (r = 0.9998) 0.0822-0.822μg (r = 0.9997), 0.0701-0.701μg (r = 0.9998), 0.0461-0.461μg (r = 0.9997) and 0.0482-0.482μg (r = 0.9998) .The average recoveries (n = 6) 97.62%, 97.81%, 97.16%, 97.32%, 96.55%. Conclusion: The method is simple, reliable and specific, and can be used as a method to control the quality of dripping pills in HUPU Wen Zhong.