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目的研究白细胞介素-6(IL-6)、血浆纤溶酶原激活物抑制剂1(PAI-1)、视黄醇结合蛋白4(RBP-4)水平在非酒精性脂肪肝(NAFLD)、肥胖儿童血清中的变化,为儿童NAFLD血清学标志物的发现提供依据。方法采用双抗体夹心酶联免疫吸附法,测定儿童血清IL-6、PAI-1、RBP-4水平。结果体重正常组与肥胖组IL-6、PAI-1水平差异均无统计学意义(P值均>0.05),体重正常组RBP-4水平显著高于肥胖组(P<0.01);NAFLD组的IL-6水平明显高于非NALFD组(P<0.01),NAFLD组的RBP-4水平显著低于非NAFLD(P<0.01);未见NAFLD组与非NAFLD组之间PAI-1水平存在差异(P>0.05)。NAFLD分度与IL-6水平呈正相关(β=0.168,P=0.013),与RBP-4呈负相关(β=-0.262,P=0.001)。结论肥胖儿童RBP-4水平低于正常体重儿童;IL-6及RBP-4与儿童NAFLD有关,可能在儿童脂肪肝的发病过程中起一定作用。
Objective To investigate the expression of interleukin-6 (IL-6), plasminogen activator inhibitor 1 (PAI-1) and retinol binding protein 4 (RBP-4) in non-alcoholic fatty liver disease (NAFLD) , Changes in serum of obese children provide the basis for the detection of NAFLD serological markers in children. Methods Serum levels of IL-6, PAI-1 and RBP-4 were measured by double antibody sandwich enzyme-linked immunosorbent assay. Results There was no significant difference in IL-6 and PAI-1 levels between normal weight group and obesity group (P> 0.05). RBP-4 level in normal weight group was significantly higher than that in obese group (P <0.01) The level of IL-6 in NAFLD group was significantly higher than that in non-NAFLD group (P <0.01). The level of RBP-4 in NAFLD group was significantly lower than that in non-NAFLD group (P <0.01) (P> 0.05). There was a positive correlation between NAFLD index and IL-6 level (β = 0.168, P = 0.013) and negative correlation with RBP-4 (β = -0.262, P = 0.001). Conclusions RBP-4 levels in obese children are lower than those in normal weight children. IL-6 and RBP-4 are associated with children’s NAFLD and may play a role in the pathogenesis of fatty liver in children.