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目的 研究乙型肝炎后肝硬化患者血清中抗 β2 糖蛋白I抗体 (aβ2 GPⅠ )与肝细胞外基质的相关性。方法 选择明确诊断的慢性乙型肝炎 (CHB)和乙型肝炎后肝硬化 (PHBC)患者为研究对象 ,以纯化的人血清β2 GPⅠ为抗原 ,用酶联免疫方法检测这些患者血清中的aβ2 GPⅠ水平 ,并且同时检测这些患者血清中的Ⅳ型胶原(Ⅳ -C)、透明质酸 (HA)和层粘连蛋白 (LN)。结果 发现PHBC患者血清中aβ2 GPⅠ水平与对照组比较有明显升高 (P <0 0 1) ,并发现PHBC患者血清中aβ2 GPⅠ高于CHB组 (P <0 0 5 )。同时发现PHBC患者血清中aβ2 GPⅠ的升高程度与肝功能分级无关 ,而与肝细胞外基质HA、Ⅳ -C、LN的血清水平呈明显正相关。结论 PHBC患者血清中aβ2 GPⅠ水平的升高 ,不仅与肝细胞受损有关 ,更与肝纤维化的细胞外基质有关
Objective To study the relationship between anti-β2 glycoprotein I antibody (aβ2 GPⅠ) and extrahepatic extracellular matrix in patients with posthepatitic cirrhosis. Methods Patients with chronic hepatitis B (CHB) and posthepatitic cirrhosis (PHBC) with definite diagnosis were enrolled in this study. Purified human serum β2 GP Ⅰ was used as antigen to detect serum aβ2 GPⅠ (IV-C), hyaluronic acid (HA) and laminin (LN) in the sera of these patients at the same time. The results showed that the level of aβ2 GPⅠ in serum of PHBC patients was significantly higher than that of the control group (P <0.01). The serum aβ2 GPⅠ level in PHBC patients was higher than that in CHB group (P <0.05). At the same time, it was found that the level of aβ2 GPⅠ in serum of PHBC patients was not correlated with the grade of liver function, but positively correlated with the serum levels of extrahepatic extracellular matrix HA, Ⅳ -C, LN. Conclusions The elevation of aβ2 GPⅠ level in serum of PHBC patients is not only related to the damage of hepatocytes, but also to the extracellular matrix of hepatic fibrosis