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为探讨广东地区汉族慢性乙型肝炎病毒患者IFN治疗 6个月Th1 /Th2因子的表达水平与HLA DRB1 0 7的相关性。收集 89例广东地区汉族接受IFN α正规治疗前和治疗 6个月慢性乙肝患者新鲜抗凝血各 5ml,通过序列特异性引物套式PCR(PCR SSP )方法进行HLA DRB1 0 7检测 ,并同时用双抗体夹心法检测患者外周血CD4+ T细胞分泌IFN γ和IL 4的水平。结果显示 ,所有患者经IFN α治疗 6个月后IFN γ水平增高 ,IL 4水平下降。HLA DRB1 0 7阳性患者治疗前后IFN γ浓度差异平均为 1 87 48pg/ml± 2 8 84pg/ml,IL 4浓度差异平均为 50 91pg/ml± 1 5 32pg/ml;HLA DRB1 0 7阴性患者治疗前后IFN γ浓度差异平均为 455 38pg/ml± 35 41pg/ml,IL 4浓度差异平均为 90 99pg/ml± 1 7 76pg/ml。慢性乙肝患者接受IFN治疗前后Th1 /Th2因子表达的变化水平与HLA DRB1 0 7位点相关
To investigate the relationship between the expression level of Th1 / Th2 and HLA-DRB1 0 at 6 months in patients with chronic hepatitis B in Han nationality in Guangdong province. A total of 89 Chinese Han patients in Guangdong were enrolled in the study. HLA-DRB107 was detected by sequence-specific PCR (PCR SSP) in 5 ml of fresh anticoagulants before and after 6 months treatment of chronic hepatitis B patients. The levels of IFN-γ and IL-4 secreted by CD4 + T cells in peripheral blood of patients were detected by double antibody sandwich method. The results showed that all patients IFN IFN α 6 months after treatment IFN γ levels increased, IL 4 levels decreased. The difference of IFNγ concentration before and after HLA DRB1 0 7 treatment was 1 87 48 pg / ml ± 2 8 84 pg / ml on average, and the difference of IL 4 concentration was 50 91 pg / ml ± 1 5 32 pg / ml on average; HLA DRB1 0 7 negative patients The difference in IFNγ concentration before and after treatment was 455 38 pg / ml ± 35 41 pg / ml on average, and the difference in IL 4 concentration was 90 99 pg / ml ± 1 7 76 pg / ml on average. The level of Th1 / Th2 expression in patients with chronic hepatitis B before and after IFN treatment was correlated with HLA DRB107