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目的:通过研究金欣口服液对呼吸道合胞病毒(respiratory syncytial virus,RSV)感染大鼠肺组织细胞因子信号抑制因子1/3(suppressor of cytokine signaling1/3,SOCS1/3)蛋白的干预作用,探讨其抗RSV感染的可能机制。方法:SD大鼠70只,随机分为正常组,感染模型组,金欣口服液低、中、高剂量组(3.3,9.9,29.7 g·kg~(-1)),预防组(提前2 d ig金欣口服液中剂量),利巴韦林组(24.5 g·kg~(-1)),每组10只,除正常组外,滴鼻感染24 h造模,给药组每天给药2次,连续给药3 d。采用酶联免疫吸附测定(ELISA)法检测RSV感染大鼠体内Ⅰ型干扰素IFN-α和IFN-β含量,逆转录-聚合酶链式反应(RT-PCR)法检测RSV感染大鼠肺组织SOCS1,SOCS3 mRNA表达量的变化,苏木素-伊红(HE)染色观察大鼠肺组织病理学变化。结果:与正常组比较,模型组RSV感染大鼠体内IFN-α和IFN-β含量明显升高,大鼠肺组织SOCS1,SOCS3 mRNA表达量明显升高(P<0.05,P<0.01),模型组大鼠肺组织病变较为明显;预防组和金欣口服液中剂量组IFN-α的表达量高于模型组(P<0.05),预防组、金欣口服液中剂量组、利巴韦林组IFN-β的表达量高于模型组(P<0.01),金欣口服液高剂量组IFN-β的表达量高于模型组(P<0.05),金欣口服液中、高剂量组SOCS1表达量低于模型组(P<0.05),金欣口服液高剂量组和利巴韦林组SOCS3表达量高于模型组(P<0.05),金欣口服液中剂量组SOCS3表达量高于模型组(P<0.01)。结论:金欣口服液可上调RSV感染大鼠体内Ⅰ型干扰素的表达,并通过抑制SOCS1/3的表达,发挥抗病毒作用,推测其为金欣口服液抗RSV感染的机制之一。
Objective: To investigate the effects of Jinxin oral liquid on the expression of suppressor of cytokine signaling1 / 3 (SOCS1 / 3) in lung tissue of rats infected with respiratory syncytial virus (RSV) Explore its possible mechanism of anti-RSV infection. Methods: Seventy SD rats were randomly divided into normal group, model group, Jinxin oral low, medium and high dose group (3.3, 9.9, 29.7 g · kg -1), preventive group d ig Jinxin oral liquid dose), ribavirin group (24.5 g · kg -1), each group of 10, except the normal group, intranasal infection 24 h modeling, the treatment group given daily Medicine 2 times, continuous administration 3 d. The levels of IFN-|Ã and IFN-|Ã were detected by enzyme linked immunosorbent assay (ELISA) in rats infected with RSV, and the lung tissues of RSV-infected rats were detected by reverse transcription-polymerase chain reaction The changes of SOCS1 and SOCS3 mRNA expression were observed. The pathological changes of lung tissue were observed by hematoxylin and eosin (HE) staining. Results: Compared with the normal group, the content of IFN-α and IFN-β in the model group was significantly increased, and the expression of SOCS1 and SOCS3 mRNA in the lung tissue of the model group was significantly increased (P <0.05, P <0.01) Compared with the model group, the expression of IFN-α in the prophylaxis group and the Jinxin oral solution group was significantly higher than that in the model group (P <0.05). The prophylaxis group, Jinxin oral liquid middle dose group, ribavirin The expression of IFN-β was higher in model group than in model group (P <0.01). The expression of IFN-β in high-dose Jinxin oral solution group was higher than that in model group (P <0.05) (P <0.05). The expression of SOCS3 in Jinxin oral liquid high-dose group and ribavirin group was higher than that in model group (P <0.05). The expression of SOCS3 in Jinxin oral liquid medium dose group was higher than that in model group Model group (P <0.01). CONCLUSION: Jinxin Oral Liquid can up-regulate the expression of type I interferon in rats infected with RSV and play an anti-viral role by inhibiting the expression of SOCS1 / 3, suggesting that it is one of the mechanisms of anti-RSV infection of Jinxin Oral Liquid.