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目的观察STERIS VHP 1000ED过氧化氢汽化低温灭菌器对生物安全实验室消毒效果。方法采用化学指示剂和生物指示剂方法,对该灭菌器用于生物安全实验室空气和物体表面消毒效果进行了检测。结果采用过氧化氢低温灭菌器,保持实验室温度在常温下,相对湿度40%~50%,空间分布过氧化氢浓度约640 mg/m3,作用90 min,所有化学指示卡均由蓝色变为米黄色,呈现合格的颜色。在相同条件下,连续4次生物检测,所有嗜热脂肪芽孢杆菌生物指示剂培养均呈阴性,所有试验组接种枯草杆菌黑色变种芽孢的平板无菌生长,阳性对照生长正常,生物监测全部合格。结论该过氧化氢低温灭菌系统对实验室内空气和物体表面具有良好消毒效果,使用操作安全。
Objective To observe the disinfection effect of STERIS VHP 1000ED hydrogen peroxide vaporization cryogenic sterilizer on biosafety laboratories. Methods The chemical indicators and biological indicators were used to detect the sterilizing effect of the sterilizer on the surface of air and objects in biosafety laboratories. Results The hydrogen peroxide cryogenic sterilizer was used to maintain the laboratory temperature at room temperature, relative humidity 40% ~ 50%, the spatial distribution of hydrogen peroxide concentration of about 640 mg / m3, the role of 90 min, all the chemical indicators by the blue Become beige, showing qualified color. Under the same conditions, four consecutive bioassays and all B. stearothermophilus bioassays were negative. All experimental groups inoculated with B. subtilis had aseptic growth of the plate, the positive control grew normally, and the biological monitoring was all qualified. Conclusion The hydrogen peroxide sterilization system on the surface of the air and objects in the laboratory has a good disinfection effect, the use of safe operation.