[Objective]To establish the HPLC fingerprint of flavonoids from SEMEN TRIGONELLAE extracts.[Methods]ZORBAX SB-C18chromatographic column(4.6 mm×250 mm,5μm)was adopted;based on gradient elution,mobile phase was methanol-0.4%phosphoric acid solution.Flow rate was 0.8 mL/min;detection wavelength was 339 nm;and injection volume was 10μL.A total of 10 batches of SEMEN TRIGONELLAE extracts were measured by HPLC with vitexin as the reference substances.[Results]The common mode of the HPLC fingerprints was established;and a total of 11 common peaks were marked.The similarity degrees of the 10 batches of samples were all greater than0.9.[Conclusions]This method was simple,accurate and repeatable,and provided scientific basis for the quality control standard and quality evaluation of SEMEN TRIGONELLAE extracts. [Objective] To establish the HPLC fingerprint of flavonoids from SEMEN TRIGONELLAE extracts. [Methods] ZORBAX SB-C18 chromatography column (4.6 mm × 250 mm, 5 μm) was adopted; based on gradient elution, mobile phase was methanol-0.4% phosphoric acid solution The flow rate was 0.8 mL / min; detection wavelength was 339 nm; and injection volume was 10 μL. A total of 10 batches of SEMEN TRIGONELLAE extracts were measured by HPLC with vitexin as the reference substances. [Results] The common mode of the HPLC fingerprints was established; and a total of 11 common peaks were marked. The similarity degrees of the 10 batches of samples were all greater than 0.9. [Conclusions] This method was simple, accurate and repeatable, and scientific basis for the quality control standard and quality evaluation of SEMEN TRIGONELLAE extracts.