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为研究前列腺素(PG)E_2对妊娠不同阶段的牛胎盘体外分泌孕酮(P)的影响,分别收集了9只妊娠270±4天及200±4天的Brahman母牛的胎盘瘤。组织切片后,与不同剂量的PGE_2、吲哚美辛、甲氯灭酸、8-表-PGE_2、8-表-PGF_(2α)、天花粉蛋白、妊娠特异性蛋白B(PSPB)或雌二醇-17β(E_2-17β)分别孵育4h或8h。用反相高效液相色谱法和酶免疫法分别测定培养液中P、PGE、PGF_(2α)、E_2-17β或PSPB的值。用ANOVA、Student-Newman-Kuehl’s检验及X~2分析进行统计学处理。
To investigate the effect of prostaglandin (PG) E_2 on in vitro secretion of progesterone (P) from bovine placenta in different stages of pregnancy, 9 Brahman cows with placenta of 270 ± 4 days and 200 ± 4 days of gestation were collected. Tissue sections were incubated with different doses of PGE 2, indomethacin, meclofenamate, 8-epi-PGE 2,8-epi-PGF 2α, trichosanthin, PSPB or estradiol -17β (E_2-17β) were incubated for 4h or 8h respectively. The contents of P, PGE, PGF 2α, E 2-17β or PSPB in the culture medium were determined by RP-HPLC and enzyme immunoassay respectively. Statistical analysis was performed using ANOVA, Student-Newman-Kuehl’s test and X-2 analysis.