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为明确抗多菌灵草莓枯萎病菌(ZY-R)菌株的生理生化特性,分析其可能的抗性机制,以课题组获得的抗多菌灵达53.91倍的ZY-R菌株及其敏感菌株(ZY)为研究材料,比较了ZY-R与ZY在苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)活力及相对渗率等方面的差异。结果表明:以清水和不同浓度多菌灵处理两菌株1.5～24 h后,ZY-R体内的POD和PAL活力始终高于ZY的;敏、抗菌株间POD活力的差异远大于PAL。在处理时间内,PAL活力先上升后下降,6 h达到最高值;而POD活力一直处于上升状态,24 h达到最高值,且敏、抗菌株之间的差异达到最大,25 mg/L条件下ZY-R的POD活力为ZY的2.26倍;此外,与ZY相比,ZY-R可以渗出更多的电解质。
In order to clarify the physiological and biochemical characteristics of the resistant strain ZY-R and to analyze its possible mechanism of resistance, the ZY-R strain with 53.91-fold resistance to carbendazim and its sensitive strain ( ZY) were used as materials to compare the differences between ZY-R and ZY in the activity and relative permeability of phenylalanine ammonia lyase (PAL) and peroxidase (POD). The results showed that the activities of POD and PAL in ZY-R were always higher than those in ZY after treated with water and different concentrations of carbendazim for 1.5-24 h. The differences in POD activity between the two strains were much greater than PAL. During the treatment time, the activity of PAL increased first and then decreased, reaching the highest value at 6 h. However, the activity of POD kept rising until the highest value reached at 24 h, and the difference between sensitive and anti-bacterial strains reached the maximum. Under the condition of 25 mg / L POD activity of ZY-R was 2.26 times that of ZY. In addition, ZY-R could exude more electrolytes than ZY-R.