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将SD大鼠随机分成四组:空白对照组(NC);ALN干预组(AL);高脂饲料喂养组(HF);高脂饲料和ALN干预组(AH)。检测第1、5、9、13、17周末大鼠血清OCN、FBG、APN和INS。采用实时定量聚合酶链反应(RTQ-PCR)检测第17周末各组大鼠股骨头骨组织OCNmRNA表达量。结果:HF组和AH组于第9周始出现FBG和血清INS升高(P<0.05),但AH组第17周INS未升高并且较13周降低(P<0.01),与之相反测得的FBG比13周增高(P<0.05)。血清APN和OCN出现相同的变化情况,AH组第13周出现降低(P<0.05),第17周降低更为明显(P<0.01);HF组仅于第17周出现降低(P<0.05)。AL组和AH组OCNmRNA的表达均明显增高(P<0.01),并且AL组和AH组与HF组比较均表达增加(P<0.01)。结论:ALN可以增加骨组织OCNmRNA表达,但肥胖与骨组织中的OCNmRNA表达无明显关系。在长时间的ALN干预和基于肥胖的条件下可使血清OCN浓度降低,从而可能影响INS分泌及糖代谢。
The SD rats were randomly divided into four groups: blank control group (NC), ALN intervention group (AL), high fat diet feeding group (HF), high fat diet and ALN intervention group (AH). Serum OCN, FBG, APN and INS were detected at the end of the 1st, 5th, 9th, 13th and 17th weeks. Real-time quantitative polymerase chain reaction (RTQ-PCR) was used to detect OCN mRNA expression in the femoral head bone of the rats in the 17th week. Results: In the HF group and the AH group, FBG and serum INS increased at the 9th week (P <0.05). However, INS at 17th week in AH group was not increased and decreased at 13th week (P <0.01) FBG was higher than 13 weeks (P <0.05). Serum APN and OCN showed the same changes at the 13th week in AH group (P <0.05), and decreased significantly at 17th week in AH group (P <0.01) . The expression of OCN mRNA in AL group and AH group were significantly increased (P <0.01), and the expression of OCN mRNA in AL group and AH group increased compared with HF group (P <0.01). CONCLUSION: ALN can increase the expression of OCN mRNA in bone tissue, but there is no significant correlation between obesity and OCN mRNA expression in bone tissue. Long-term ALN intervention and obesity-based conditions can reduce the concentration of serum OCN, which may affect INS secretion and glucose metabolism.