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为研究乳腺上皮细胞增值和分化特性,通过有效的培养方法,实现了牛乳腺上皮细胞的体外培养,并且所获细胞具有正常的生理特性及功能。本文综述了近年来关于牛乳腺上皮细胞体外分离、培养的研究进展。并对牛乳腺上皮细胞在不同培养体系中的生长状态、分化差异;牛乳腺上皮细胞对各种激素和生长因子的应答进行了讨论。
In order to study the proliferation and differentiation of mammary epithelial cells, the cultured bovine mammary epithelial cells were cultured in vitro and the cells had normal physiological characteristics and functions. This review summarizes recent research progress on the isolation and culture of bovine mammary epithelial cells in vitro. Differences in the growth and differentiation of bovine mammary epithelial cells in different culture systems were also discussed. The responses of bovine mammary epithelial cells to various hormones and growth factors were also discussed.