参照酚—水法从马流产杆菌中提取出脂多糖,其纯度达到参考标准品的要求。用它对早、中和晚期妊娠小鼠进行腹腔或尾静脉注射,每只小鼠(体重25—30克)注射剂量在5—30μg均能产生终止妊娠的效果,有效率达93—100%。每只注射剂量25和25μg以下的妊娠小鼠全部存活,无明显毒性反应,而30μg剂量的8只小鼠中有2只死亡。 According to the phenol-water method, lipopolysaccharides were extracted from B. abortus, the purity of which meets the requirements of reference standards. It is used to administer intraperitoneal or caudal vein injections to early, mid and late pregnancy mice. Each mouse (body weight 25-30 g) can produce an effect of termination of pregnancy at an injection dose of 5-30 μg, with an effective rate of 93-100%. . All of the mice immunized with injection doses of 25 and 25 μg or less survived without significant toxicity, while 2 of the 8 mice in the 30 μg dose died.