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目的 探讨辐射对培养小鼠骨髓基质细胞内核因子 κB(NF κB)的影响。方法 采用免疫组化法及凝胶电泳迁移率实验。结果 免疫组化方法显示正常骨髓基质细胞核的周围表达低水平的NF κB ,8.0Gy60 Co辐射损伤后 1h即见其表达较正常显著升高并出现明显的核移位 ,4h达高峰 ,6h有所回落 ,8h恢复正常 ;凝胶电泳迁移率实验发现正常骨髓基质细胞内存在低水平活性NF κB ,8.0Gy60 Co辐射损伤后 1h即见其活性明显升高 ,4h达高峰 ,8h恢复正常。结论 8.0Gy辐射损伤后培养骨髓基质细胞内NF κB的表达升高并激活 ,这可能与骨髓微环境内基质细胞的抗辐射能力及造血功能的恢复有关。
Objective To investigate the effect of radiation on the expression of nuclear factor κB (NF κB) in cultured mouse bone marrow stromal cells. Methods Immunohistochemistry and gel electrophoretic mobility shift assay were used. Results Immunohistochemistry showed that the expression of NF-κB was low in the normal nuclei of the bone marrow stroma cells. The expression of NF-κB was significantly increased at 1 h after the 8.0 Gy60 Co radiation injury and reached a peak at 4 h after 6 h After 8h, the activity returned to normal after 8h. The activity of NF-κB in normal bone marrow stromal cells was found to be low in normal bone marrow stromal cells. The activity of NFκB was significantly increased at 1h after 8.0Gy60Co radiation injury, reaching the peak at 4h and returned to normal at 8h. Conclusion The expression of NF-κB in cultured bone marrow stromal cells after 8.0Gy radiation injury is increased and activated, which may be related to the anti-radiation ability of stromal cells in the bone marrow microenvironment and the recovery of hematopoietic function.