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目的:研究E-钙黏附蛋白(E-cadherin,E-cad)mRNA在正常卵巢、上皮性卵巢良性肿瘤及上皮性卵巢癌组织中的表达,并探讨E-cad表达与上皮性卵巢癌临床病理特征的关系。方法:用荧光定量-PCR法(FQ-PCR)分别检测正常卵巢上皮组织、卵巢上皮性良性肿瘤以及上皮性卵巢癌组织中E-cad的表达水平。结果:E-cad在10例正常卵巢上皮组织中表达为0,阳性表达率0;在10例良性卵巢上皮性肿瘤组织中阳性表达为0.261 7±0.138 2,阳性表达率为100%;在30例恶性卵巢上皮性肿瘤组织中阳性表达为0.012 7±0.018 1,阳性表达率为50%;3组间比较差异有统计学意义,χ2=30.924,P<0.05。E-cad的表达与FIGO分期成负相关,r=-0.873,P<0.05;与患者的生存时间呈正相关,r=0.602,P<0.05。E-cad表达与其组织学类型无相关性,χ2=3.811,P>0.05;与病理分级无相关性,χ2=3.458,P>0.05。结论:E-cad可作为上皮性卵巢癌病情进展和预后判断的指标之一,为分子靶向治疗提供一个依据。
Objective: To investigate the expression of E-cadherin (E-cad) mRNA in normal ovarian epithelial ovarian epithelial ovarian cancer and epithelial ovarian cancer, and to explore the relationship between the expression of E-cad and epithelial ovarian cancer Characteristics of the relationship. Methods: The expression of E-cad in normal ovarian epithelial tissue, benign epithelial ovarian tumor and epithelial ovarian cancer was detected by fluorescence quantitative-PCR (FQ-PCR). Results: The positive expression rate of E-cad was 0 in 10 cases of normal ovarian epithelial tissue, and the positive expression rate was 0.261 7 ± 0.138 2 in 10 cases of benign epithelial ovarian tumor. The positive rate of E-cad was 30% The positive expression rate of malignant ovarian epithelial tumor was 0.012 7 ± 0.018 1, the positive rate was 50%. The difference between the three groups was statistically significant (χ2 = 30.924, P <0.05). The expression of E-cad was negatively correlated with the FIGO staging, r = -0.873, P <0.05; it was positively correlated with the patient’s survival time, r = 0.602, P <0.05. There was no correlation between the expression of E-cad and its histological type, χ2 = 3.811, P> 0.05; there was no correlation with the pathological grade, χ2 = 3.458, P> 0.05. Conclusion: E-cad may be one of the indicators of the progression of disease and prognosis of epithelial ovarian cancer, providing a basis for molecular targeted therapy.