本文采用聚丙烯酰胺凝胶电泳的方法，对利用花粉管通道途径，导入外源ＤＮＡ所获大豆变异后代，进行过氧化物酶酶谱分析。结果表明：变异后代与亲本的谱带间有明显差异，后代含有供体的谱带，这说明了外源ＤＮＡ片段已整合到受体基因组中，并在后代中得到了表达。因此，我们认为可以用同工酶酶谱分析的结果作为鉴定外源ＤＮＡ导入大豆的生化指标之一。 In this paper, polyacrylamide gel electrophoresis method, the use of pollen tube pathway, the introduction of foreign DNA obtained by mutation of soybean offspring, peroxidase zymography. The results showed that there was a significant difference between the bands of the offspring and the parents, and the offspring contained donor bands, indicating that the foreign DNA fragments had been integrated into the recipient genome and expressed in the offspring. Therefore, we think that isozyme zymogram analysis can be used as one of the biochemical indexes to identify foreign DNA introduction into soybean.