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目的探究化学法与气相色谱法测定毒鼠强出现假阳性的原因及解决方法,以提高基层对毒鼠强中毒检测的准确性,为临床实施正确的救治提供可靠依据。方法将已知样品处理后,分别用基层常用的化学法与气相色谱法进行毒鼠强定性测定,核查两种方法的准确性。结果化学法与气相色谱法两种方法的测定结果与已知结果进行比较,气相色谱法的假阳性较化学法少,但也存在假阳性问题。结论在运用化学法和气相色谱法定性测定毒鼠强出现阳性结果时,化学法测定的阳性样品应再进行高温处理,以排除假阳性;气相色谱法测定的阳性样品要改变色谱柱的分离条件或采用双色谱柱分离测定,每次测定在保留时间均与毒鼠强标准样品一致时,才可定性为毒鼠强中毒。
Objective To explore the causes and solutions of determining the strong positive of tetramine by chemical method and gas chromatography in order to improve the accurate detection of tetramine poisoning in grass roots and provide a reliable basis for the correct clinical treatment. Methods After the known samples were treated, the determination of tetramine in rats was carried out by chemical methods commonly used in the grass roots and gas chromatography respectively, and the accuracy of the two methods was verified. Results The results of two methods of chemical method and gas chromatography were compared with the known results. The false positive of GC was less than that of chemical method, but there was also the problem of false positive. Conclusion In the qualitative determination of tetramine by chemical method and gas chromatography, the positive samples determined by chemical method should be treated with high temperature to eliminate the false positives. The positive samples determined by gas chromatography should change the separation conditions Or the use of dual-column separation assay, each determination of the retention time are consistent with tetramine strong standard samples, can be characterized as tetramine poisoning.