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目的:探讨克感复方在MDCK细胞上抗流感病毒的作用并对其机制进行初步研究。方法:以利巴韦林为阳性对照药物,运用抑制试验、预防试验、中和试验3种方法,以MTT法检测细胞存活率,研究克感复方抗流感病毒的作用并判断其作用原理。同时,通过药物血凝抑制实验分析其抗流感病毒作用是否与病毒血凝素相关。结果:克感复方对MDCK细胞的半数毒性浓度(CC50)为(2.84±0.65)mg/mL,抑制流感病毒增殖的半数有效浓度(EC50)为(0.18±0.12)mg/mL,选择性指数(SI)为15.78。有细胞外杀流感病毒作用,但作用较弱,最高抑制率未超过50%。无阻断流感病毒吸附的作用。无药物血凝抑制作用。结论:克感复方在MDCK细胞上具有抗流感病毒的作用,其中抑制流感病毒增殖的能力最强。
OBJECTIVE: To investigate the anti-influenza virus effect of Kegan compound on MDCK cells and to study its mechanism. Methods: Ribavirin was used as a positive control drug. Inhibition test, prophylactic test and neutralization test were used to detect the cell viability by MTT assay. The effect of anti-influenza virus was evaluated and the principle of action was determined. At the same time, through the drug hemagglutination inhibition experiment analysis whether the anti-influenza virus effect is associated with the virus hemagglutinin. Results: The median lethal concentration (CC50) of MDRK on MDCK cells was (2.84 ± 0.65) mg / mL, and the EC50 of influenza virus inhibition was (0.18 ± 0.12) mg / mL. The selectivity index SI) is 15.78. There are extracellular killing influenza virus effect, but the role of weak, the highest inhibition rate did not exceed 50%. No blocking effect of influenza virus adsorption. No drug hemagglutination inhibition. Conclusion: Kegan compound has the anti-influenza virus effect on MDCK cells, of which the ability to inhibit the proliferation of influenza virus strongest.