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沙枣皮经用水超声浸取可得呈黄色的沙枣皮色素提取液。将此提取液经装有AB-8吸附树脂的固相萃取柱净化,用乙醇-水(7+3)溶液作淋洗液从柱上洗脱该色素,并用真空蒸发得到具有荧光性质的色素溶液。由于铁(Ⅲ)与沙枣皮色素的结合反应,引起沙枣皮色素荧光淬灭,且其荧光淬灭程度与铁(Ⅲ)浓度在2.0×10~(-6)～6.0×10~(-5)mol·L~(-1)范围内呈线性关系。据此提出了以沙枣皮色素为探针测定铁(Ⅲ)的荧光光度法。方法的检出限(3S/N)为1.0×10~(-7)mol·L~(-1)。用标准加入法对方法的回收率作了试验,测得回收率在99.5%～101.5%之间。
Sassafras patensis by ultrasonic leaching of water can be obtained yellow Cassia bark skin pigment extract. The extract was purified by a solid-phase extraction cartridge equipped with an AB-8 adsorption resin, eluting the dye from the column with an ethanol-water (7 + 3) solution and evaporating in vacuo to obtain a dye having a fluorescent property Solution. Due to the binding reaction between iron (Ⅲ) and Phellodendron bark pigments, it caused the fluorescence quenching of Phellodendri chinensis fruit, and its fluorescence quenching degree and iron (Ⅲ) concentration ranged from 2.0 × 10 -6 to 6.0 × 10 ~ -5) mol·L ~ (-1) within a linear range. Based on this, a new fluorimetric method was developed for the determination of iron (Ⅲ) with Psidium camaraya pigment. The detection limit (3S / N) of the method was 1.0 × 10 -7 mol·L -1. The recovery rate of the method was tested by the standard addition method, and the recovery rate was between 99.5% and 101.5%.