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用普通小麦的幼胚在Ｎ６Ｂ５ＭＳⅠ培养基上诱导出胚性和非胚性愈伤组织，将这两种愈伤组织分别转至Ｎ６Ｂ５ＭＳⅡ培养基后，胚性愈伤组织形成许多体细胞胚，非胚性愈伤组织形成不定苗。胚性愈伤组织转入Ｎ６Ｂ５ＭＳⅡ培养基后，第２～４ｄ形成胚性细胞，二细胞、四细胞原胚，８ｄ后，则以多细胞原胚为主，１２～２４ｄ后，可见球形胚和梨形胚，２６ｄ后则多为成熟胚。利用放射性同位素液体闪烁计数技术结合核酸、蛋白质合成抑制剂，研究了以小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）幼胚为外植体诱导产生体细胞胚的发生、发育过程中ＤＮＡ、ＲＮＡ和蛋白质的合成动态。发现ＲＮＡ、蛋白质的合成分别在胚性愈伤组织培养后的第４和第８ｄ就达到高峰，ＤＮＡ的合成也明显增加，但变化平缓。而非胚性愈伤组织中的核酸和蛋白质的合成速率远远低于胚性愈伤组织。加入抑制剂后，不仅抑制了核酸、蛋白质的合成，同时也抑制了胚性愈伤组织的生长和体细胞胚的发生、发育，加入时间越早，抑制效应越明显。可见，ＤＮＡ、ＲＮＡ和蛋白质是体细胞胚发生、发育的分子基础。
Embryogenic and non-embryogenic callus was induced on the N6B5MSⅠmedium by immature embryos of common wheat. After the two callus were transferred to N6B5MSⅡmedium, embryogenic callus formed many somatic embryos, Embryogenic callus formation of adventitious seedlings. Embryogenic calli transformed into N6B5MS Ⅱ medium, embryo cells, two-cell and four-cell embryos were formed on the 2nd to 4th days. After 8 days, embryogenic callus was dominated by multicellular embryos. After 12 ~ 24 days, Pear-shaped embryos, mostly after 26d mature embryos. Using radioactive isotope liquid scintillation counting technique combined with nucleic acid and protein synthesis inhibitors, the synthesis and dynamics of DNA, RNA and protein during the development of somatic embryos induced by immature embryos of wheat (Triticum aestivum L.) were studied. The results showed that the synthesis of RNA and protein peaked on the 4th and 8th day respectively after embryogenic callus culture, and the DNA synthesis increased obviously, but the change was gentle. The rate of synthesis of nucleic acids and proteins in non-embryogenic callus was much lower than that in embryogenic callus. Adding inhibitors not only inhibited the synthesis of nucleic acid and protein, but also inhibited the growth of embryogenic callus and the occurrence and development of somatic embryos. The earlier the addition was, the more obvious the inhibitory effect was. Can be seen, DNA, RNA and protein somatic embryogenesis, the molecular basis of development.