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目的 探讨重离子诱发人支气管上皮细胞系的突变效应。方法 用ＨＩ１３ 串列加速器产生的Ｌｉ、Ｃ、Ｆ三种离子束以０－５～６－０ Ｇｙ 设计剂量照射人支气管上皮细胞系突变，观察其突变效应。结果 细胞ｈｐｒｔ 突变率（ ＭＦ，无量纲） 与重离子剂量（Ｄ，Ｇｙ） 的线性回归方程分别为ＭＦ＝１－６３×１０－ ５Ｄ（Ｌｉ） 、ＭＦ＝ ８－６４×１０－ ５Ｄ（Ｃ） 、ＭＦ＝ １－３３ ×１０－ ５Ｄ（Ｆ） ；与６０Ｃｏγ射线比较，其相对生物效应依次为１－２７、６－７８ 、１－０４。此突变率与粒子注量亦呈线性正相关，三种重离子的突变截面（σｍ ，１０－ ４μｍ２）依次为２－６１、４１－５ 、２１－３。致突性（σｍ／σｉ，１０－５） 依次为２－０８、１０－２、２－７８。结论 细胞突变率随重离子剂量增大而上升，似有ＬＥＴ 依赖性。
Objective To investigate the mutation effect of heavy ion-induced human bronchial epithelial cell line. Methods Three kinds of Li, C and F ion beams generated by HI13 tandem accelerator were used to irradiate the human bronchial epithelial cell line mutation at a designed dose of 0-5 ~ 6-0 Gy to observe the mutation effect. Results The linear regression equations of cell hprt mutation rate (MF, dimensionless) and heavy ion dose (D, Gy) were MF = 1-63 × 10-5D (Li) and MF = 8-64 × 10-5D ), MF = 1-33 × 10-5D (F). Compared with 60Coγ-rays, the relative biological effects were 1-27, 6-78 and 1-04. The mutation rate was also positively correlated with the particle fluence. The mutation cross sections (σm, 10-4μm2) of the three heavy ions were 2-61, 41-5 and 21-3, respectively. Mutagenesis (σm / σi, 10-5) followed by 2-08,10-2,2-78. Conclusions The rate of cell mutation increases with the increase of heavy ion dose, which seems to be LET dependent.