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以‘银玉’白萝卜小孢子胚状体为试材,研究激素对胚状体再生的影响。结果表明:在B5培养基中添加0.2mg/L 6-苄氨基嘌呤(6-BA)能够促进胚状体分化成再生植株,萘乙酸(NAA)和吲哚丁酸(IBA)可促进再生植株生根,IBA效果好于NAA,IBA适宜的质量浓度为0.5mg/L。利用流式细胞仪(Flow Cytometry,FCM)对163株小孢子再生苗的倍性进行测定,表明萝卜小孢子再生株的自然加倍率为82.21%,17.79%为单倍体,44.17%为双单倍体,38.04%为多倍体。其中多倍体包括三倍体、四倍体、八倍体和嵌合体。观察和测定不同倍性再生植株苗期和花期的植物学性状,并与供体植株进行对比。结果显示单倍体植株弱小,没有花粉;四倍体和八倍体长势强,花粉发育正常;三倍体苗期长势与双单倍体相当,但花器官弱小,药室内无花粉或有微量花粉;双单倍体与供体植株长势相同。
The effect of hormones on embryoid body regeneration was studied with the embryo of ’YinYu’ white radish microspores. The results showed that the addition of 0.2 mg / L 6- benzylaminopurine (6-BA) to B5 medium promoted the differentiation of embryoid bodies into regenerated plants. NAA and IBA promoted the regeneration of regenerated plants Roots, IBA better than NAA, IBA appropriate mass concentration of 0.5mg / L. The ploidy of 163 microspore regenerated seedlings was determined by flow cytometry (FCM), which indicated that the natural doubling rate of microspore regenerated plants was 82.21%, 17.79% was haploid and 44.17% was double single Ploidy, 38.04% of polyploidy. Among them, polyploid includes triploid, tetraploid, octaploid and chimera. The botanical characters at the seedling and flowering stages of different ploidy regenerated plants were observed and compared with that of donor plants. The results showed that haploid plants were weak and pollen-free, tetraploid and octaploid were strong, and pollen development was normal. Triploid seedlings grew well with double haploid plants, but flower organ was weak, Pollen; double haploid and donor plants grow in the same.