以牛血清白蛋白与其单克隆抗体为样品，初步研究了蛋白质的非竞争性毛细管电泳免疫分析方法。混合温育可以在对20min内达到平衡，电泳分离在12min内完成。当示踪物浓度为320nmol／L时，所得到的标准曲线的线性范围为8－150nmol／L，检出限为5nmol／L，并考察了缓冲溶液的浓度、pH、分离电压等因素的影响。 Using bovine serum albumin and its monoclonal antibody as samples, the noncompetitive capillary electrophoresis immunoassay of protein was studied. Mixed incubation can be achieved within 20min to balance, electrophoresis separation completed within 12min. When the concentration of tracer was 320 nmol / L, the linearity of the obtained standard curve was 8-150 nmol / L and the detection limit was 5 nmol / L. The effects of buffer concentration, pH, separation voltage and other factors .