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以淡绿叶标记基因系ＩＧＭ１９与籼型光－温敏核不育系８９０２５杂交，再用８９０２Ｓ回交１次，通过多代选育获得了带淡绿色叶隐性标记性状，且育性转换特性与８９０２Ｓ明显不同的籼型光－温敏核不育系Ｍ２Ｓ。在杭州（３０°０５’Ｎ）自然条件下，Ｍ２Ｓ具有连续３０ｄ以上的不育期（不育度＞９９．５％），在秋季正常情况下，结实率可达５１．８％。人工气候箱控制条件下，Ｍ２Ｓ在短日适温时结实正常，在长日（１５．０ｈ）与所有处理温度（３０．１℃、２４．１℃和２３．１℃）、高温（３０．１℃）与所有处理日长（１５．０ｈ、１４．０ｈ和１２．５ｈ）的光温组合下均完全不育或高度不育。结实率的方差分析结果表明，其育性转换的温度效应和光周期效应均不显著，仅光温工作效应显著，属典型的光温互作雄性不育系。Ｍ２Ｓ本身较稳定的不育性和带有隐性淡绿色叶的标记性状将有利于在两系法杂交水稻中应用。
Light green leaf marker gene IGM19 was crossed with indica thermo-thermo-sensitive genic male sterile line 89025 and then backcrossed once with 8902S. The recessive green leaf trait marker was obtained through multigenerational selection, and the fertility transformation characteristics Indica photo-thermo-sensitive genic male sterile line M2S distinctly different from 8902S. Under the natural conditions of Hangzhou (30 ° 05’N), M2S had a sterile period of more than 30 days (sterility> 99.5%), and its seed setting rate was 51.8% under normal conditions in autumn. Under the control of artificial climate chamber, M2S was normal in the short day, with all treatment temperatures (30.1 ℃, 24.1 ℃ and 23.1 ℃) and high temperature (30 ℃) in the long day (15.0h) 1 ℃) and all the light-temperature combinations of treatment day length (15.0h, 14.0h and 12.5h) were completely sterile or highly sterile. Analysis of variance of seed setting rate showed that both temperature effect and photoperiod effect of fertility transformation were not significant, and only light-temperature work effect was significant, which was a typical light-temperature interaction male sterile line. The more stable sterility of M2S and the marker trait with recessive pale green leaf will be helpful for the application of two-line hybrid rice.