论文部分内容阅读
ＭＳ培养基中添加镧（Ｌａ）（０ ．０１ ～０ ．１０ｍ ｍｏｌ·Ｌ－ １） 培养烟草愈伤组织，其总Ｃａ２ ＋ 及原生质体Ｃａ２ ＋ 含量明显比对照（ 不加Ｌａ） 低；长时间（ 继代培养３０ｄ） 较短时间培养（ 悬浮培养２４ｈ） 低．低浓度（０ ．０１ ～０ ．０５１ｍｏｌ·Ｌ－ １）Ｌａ３ ＋ 使细胞壁中Ｃａ２ ＋ 含量高于对照并随浓度提高而增加，高浓度（０ ．１０１ｍｏｌ·Ｌ－ １） 则减少．与Ｃｅ２ ＋ 相比较，Ｌａ３ ＋ 的排Ｃａ２ ＋ 作用稍弱于Ｃｅ３ ＋ ．以不同浓度Ｌａ（ＮＯ３）３ 溶液浸种，油菜幼根总Ｃａ２ ＋ 及原生质体中Ｃａ２ ＋ 含量变化与上述类似．Ｌａ３ ＋ 使烟草愈伤组织褐化明显，生长量比对照低．
MS medium added lanthanum (La) (0. 01 ~ 0. 10m mol·L-1) cultured tobacco callus, the total Ca2 + and protoplasts Ca2 + content was significantly lower than the control (without La); long Time (subculture 30d) culture for a short time (suspension culture 24h) low. Low concentration (0 .01 ~ 0. 051mol·L-1) La3 + increased the content of Ca2 + in the cell wall and increased with the increase of concentration, but decreased at high concentration (0.101mol·L-1). Compared with Ce2 +, Ca2 + emission of La3 + is weaker than that of Ce3 +. The soaking of Ca (superscript 3 +) with different concentrations of La (NO3) 3 showed that Ca2 + contents in total Ca2 + and protoplasts in rapeseed roots were similar to those above. La3 + brown tobacco callus significantly, the growth is lower than the control.