In this paper a new method is developed to make a dynamic layout adjustment and navigation for enterprise Geographic Information System(GIS) based on object mark recognition. The extraction of object mark images is based on some morphological structural patterns, which are described by morphological structural points, contour property, and other geometrical data in a binary image of enterprise geographic information map. Some pre-processing methods, contour smooth following, linearization and extraction patterns of structural points, are introduced. If any special object is selected to make a decision in a GIS map, the all information around it will be obtained. That is, we need to investigate similar object enterprises around selected region to analyse whether it is necessary for establishing the object enterprise at that place. To further navigate GIS map, we need to move from one region to another. Each time a region is formed and displayed based on the user′ s focus. If a focus point of a map is selected, in terms of extracted object mark image, a dynamic layout and navigation diagram is constructed. When the user changes the focus (i.e. click a node in the navigation mode), a new sub-diagram is formed by dropping old nodes and adding new nodes. The prototype system provides effective interfaces that support GIS image navigation, detailed local image/map viewing, and enterprise information browsing.