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采用扩增片段长度多态性 (Amplifiedfragmentlengthpolymorphism ,AFLP )技术 ,研究了B型烟粉虱Bemisiatabaci噻虫嗪(thiamethoxam )抗性品系在DNA分子水平上的遗传分化。实验数据用STATISTICA ( 4 5版本 )软件进行分析 ,遗传距离用UPGMA (非加权对组算术平均值聚类 )方法进行聚类。在聚类树中 ,首先是抗性个体与敏感个体聚为 2枝 ,然后雌、雄个体聚为 2枝 ,说明抗性品系在DNA分子水平上已存在明显分化。另外 ,利用EcoRⅠ +ACT MseⅠ +CTG引物 ,分别在全部抗性个体中扩增出了敏感个体所没有的特异性同一片段和在全部敏感个体中扩增出了抗性个体所没有的特异性同一片段 ,经克隆、测序 ,这 2个片段分别是 1 5 0bp和 3 1 5bp。此 2个片段 ,可作为烟粉虱抗噻虫嗪品系和敏感品系的分子标记而在抗性监测中得以应用
Amplified fragment length polymorphism (AFLP) technique was used to study the genetic differentiation of Bemisia tabaci resistant strains of Bemisia tabaci B at the DNA molecular level. Experimental data were analyzed using STATISTICA (version 45) software and the genetic distance was clustered using UPGMA (Unweighted Pair Arithmetic Average Means Clustering) method. In the cluster tree, first, the resistant individuals and the susceptible individuals clustered into two branches, then the female and male individuals were clustered into two branches, indicating that the resistant lines had obvious differentiation at the DNA molecular level. In addition, EcoRⅠ + ACT MseⅠ + CTG primers were used to amplify all the resistant individuals in the same region of the same individual that the sensitive individuals did not have and the same one that the resistant individuals did not amplify in all the sensitive individuals The fragments were cloned and sequenced. The two fragments were 150 bp and 351 bp, respectively. These two fragments can be used as molecular markers for resistance to thiamethoxam and sensitive strains of B. tabaci to resistance monitoring