论文部分内容阅读
目的制备聚乙二醇1000维生素E琥珀酸酯(TPGS)修饰的阿霉素脂质体并考察其对阿霉素抗肿瘤活性的增敏作用。方法用阳离子树脂吸附法测定阿霉素脂质体的包封率;MTT法测定对MCF-7和MCF-7/ADR的毒性;用荧光显微镜观察阿霉素的细胞摄取,并用HPLC测定细胞内的阿霉素含量。结果 TPGS修饰的阿霉素脂质体增加了MCF-7/ADR对阿霉素的摄取,并增强了对MCF-7和MCF-7/ADR细胞的毒性。结论 TPGS修饰脂质体能显著增强MCF-7和MCF-7/ADR对阿霉素的敏感性。
Objective To prepare liposomes modified with polyethylene glycol 1000 vitamin E succinate (TPGS) and investigate its sensitizing effect on the antitumor activity of doxorubicin. Methods The encapsulation efficiency of doxorubicin liposomes was determined by cationic resin adsorption method. The cytotoxicity of MCF-7 and MCF-7 / ADR was determined by MTT method. The cellular uptake of doxorubicin was observed by fluorescence microscopy. Of doxorubicin content. Results TPGS-modified doxorubicin liposomes increased uptake of doxorubicin by MCF-7 / ADR and enhanced toxicity to MCF-7 and MCF-7 / ADR cells. Conclusion TPGS modified liposomes can significantly enhance the sensitivity of doxorubicin to MCF-7 and MCF-7 / ADR.