目的和方法 :应用大鼠高原低氧模型及原位杂交技术和氨基酸测定法 ,研究下丘脑前生长抑素原 (PPS)mRNA表达和谷氨酸 (Glu)、天门冬氨酸 (Asp)含量的变化。 结果 :高原低氧组大鼠下丘脑Glu和Asp的含量明显增多 ,室周核、室旁核、弓状核PPS mRNA阳性神经元数目显著增加 ;而NMDA受体拮抗剂氯铵酮 ,虽然对Glu和Asp含量无明显影响 ,但可使高原低氧大鼠下丘脑PPS mRNA阳性神经元数目减少。结论 :下丘脑生长抑素可能参与了高原低氧反应 ,Glu通过NMDA受体可增强高原低氧大鼠下丘脑PPS mRNA的表达 PURPOSE AND METHODS: The hypothalamic pre-somatostatin (PPS) mRNA expression and the contents of glutamic acid (Glu) and aspartate (Asp) were determined using rat plateau hypoxia model, in situ hybridization and amino acid assay The change. Results: The levels of Glu and Asp in the hypothalamus of rats in plateau hypoxia group were significantly increased, and the numbers of PPS mRNA positive neurons in periventricular nucleus, paraventricular nucleus and arcuate nucleus were significantly increased. While NMDA receptor antagonist, Glu and Asp content had no significant effect, but the plateau hypoxic rat hypothalamus PPS mRNA positive neurons decreased. CONCLUSIONS: Hypothalamic somatostatin may be involved in the plateau hypoxia. Glu signaling through the NMDA receptor may enhance the hypothalamic PPS mRNA expression in plateau hypoxia rats