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目的研究天津某小区生活饮用水中胞外耐药基因的污染特征,阐明生活饮用水中胞外耐药基因污染规律。方法于2015年12月至2016年11月,利用自行研制的核酸吸附柱富集生活饮用水中的游离核酸,采用实时荧光定量PCR技术检测tetC、ermB、ampC、sul1、qnrA、aadA、catA1和katG 8种胞外耐药基因的浓度。结果与结论在12份水样中,tet C的检出率最高,约为66.7%;其次为sul1和qnr A(41.7%)、ampC(33.3%)、aadA(16.7%)、cat A1和kat G(均为8.33%),erm B未检测出。生活饮用水中以sul1胞外耐药基因的污染最严重,达(1.27±0.04)×10~5GC/L。各胞外耐药基因出现最大浓度的时间主要集中在6月,且3月和6月检测出的胞外耐药基因种类最多,达5种。天津某小区生活饮用水中存在多种胞外耐药基因的污染。
Objective To study the pollution characteristics of extracellular drug resistance genes in drinking water of a district in Tianjin and to clarify the rules of extracellular drug resistance in drinking water. Methods From December 2015 to November 2016, free nucleic acids in domestic drinking water were enriched by self-developed nucleic acid adsorption column. Real-time fluorescence quantitative PCR was used to detect tetC, ermB, ampC, sul1, qnrA, aadA, catA1 and katG eight kinds of extracellular resistance gene concentration. RESULTS AND CONCLUSION The highest tetracycline (66.7%) was detected in 12 samples, followed by sul1 and qnr A (41.7%), ampC (33.3%), aadA (16.7%), cat A1 and kat G (both 8.33%), erm B not detected. The most serious contamination of sul1 gene in drinking water was (1.27 ± 0.04) × 10 ~ 5GC / L. The time of maximum concentration of each extracellular drug resistance gene mainly concentrated in June, and the most extracellular drug resistance genes were detected in March and June, up to 5 kinds. There is a variety of extracellular drug resistance genes in the drinking water of a district in Tianjin.