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本文探讨食物中胆固醇的薄层光密度法的测定。用硅胶Ｇ与０．３％ＣＭＣ－Ｎａ按１：２．２比例制成薄层板，石油醚－乙醚－冰醋酸（７：３：０．１）为展开剂，５％磷钼酸乙醇溶液显色，日本岛津ＣＳ－９１０型薄层扫描仪定量扫描。测得精密度变异系数（ＲＳＤ％）为２．９２，稳定性变异系数（ＲＳＤ％）为２．３５，回收率在８７．９５～９４．２９％范围内。与气相色谱法（其回收率在９２％～１０３％范围）比较，结果相接近。该法简便易行，重现性、稳定性好。
This article explores the determination of cholesterol in food by optical densitometry. Silica Gel G and 0.3% CMC-Na 1: 2.2 ratio made of thin-layer plate, petroleum ether - ether - glacial acetic acid (7: 3: 0.1) as developing agent, 5% Solution color, Japan Shimadzu CS-910 TLC scanner quantitative scanning. The precision coefficient of variation (RSD%) was 2.92, the coefficient of variation of stability (RSD%) was 2.35, and the recovery rate was in the range of 87.95-94.29%. Compared with gas chromatography (recovery ranged from 92% to 103%), the results were similar. The method is simple, reproducible and stable.