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考察不同因素对异化铁还原菌(Dissimilatory Iron Reducing Bacteria,DIRB)还原高岭土中Fe(Ⅲ)的影响,通过在底液中添加不同有机酸、草酸盐、络合剂和重金属离子来研究微生物还原铁的机制。结果发现,添加草酸对微生物还原铁有明显的促进作用,而乙酸和水杨酸则抑制了微生物的生长;添加草酸钠的实验表明草酸对微生物的促进作用是通过草酸根离子的作用而与草酸的酸性并无联系;络合剂(NTA)可以提高微生物异化铁还原量,而EDTA不影响铁还原的作用;Cu(Ⅱ)对微生物具有毒害作用从而抑制了微生物的还原活性,Mn(Ⅱ)对铁的还原则有明显的促进作用。此外,采用logistic方程对有促进作用的曲线进行拟合,发现添加草酸钠对微生物的还原铁促进作用最大。
The effects of different factors on the reduction of Fe (Ⅲ) in kaolin by Dissimilatory Iron Reducing Bacteria (DIRB) were investigated. The effects of different organic acids, oxalates, complexing agents and heavy metal ions on the microbial reduction Iron mechanism. The results showed that the addition of oxalic acid could significantly promote the reduction of microbial iron, while acetic acid and salicylic acid inhibited the growth of microorganisms. The addition of sodium oxalate showed that oxalic acid promoted the growth of microorganisms through the interaction of oxalate with oxalic acid The complexing agent (NTA) could increase the amount of iron reduction of microorganisms, while EDTA did not affect the reduction of iron. Cu (Ⅱ) had toxic effect on microorganisms and inhibited the reduction activity of Mn (Ⅱ) The reduction of iron has a significant role in promoting. In addition, the logistic equation was used to fit the curve with the promotion effect, and it was found that the addition of sodium oxalate had the greatest promotion effect on reducing iron of the microorganism.