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为探明灰飞虱Laodelphax striatellus滞育过程中生理生化的变化特点,利用蒽酮比色法、甘油酶法、BCA法和分光光度法,测定了不同龄期非滞育、滞育灰飞虱若虫体内的总糖、糖原、海藻糖、甘油、蛋白质和氨基酸含量。结果表明,灰飞虱各龄期滞育若虫体内总糖、糖原、海藻糖、甘油、总蛋白和总氨基酸的含量与非滞育若虫存在差异。滞育若虫体内糖原和甘油的含量与非滞育若虫无显著差异,而总糖、海藻糖和氨基酸的含量均显著高于非滞育若虫,3龄滞育若虫体内海藻糖的含量为95.5μg/mg,是非滞育若虫的1.4倍;各龄期滞育若虫体内总蛋白含量均极显著高于非滞育若虫,3龄滞育若虫体内的总蛋白含量最大,为0.68 g/L,是非滞育若虫的1.3倍。研究表明,灰飞虱滞育个体属于海藻糖积累型,并通过滞育系统调节生理生化习性以增强抗逆性,从而渡过不良环境。
In order to find out the characteristics of physiological and biochemical changes of Laodelphax striatellus during diapause, anthraquinone, glycerol, BCA and spectrophotometry were used to determine the effects of non-diapause, Nymphs in the body of total sugar, glycogen, trehalose, glycerol, protein and amino acid content. The results showed that the contents of total sugar, glycogen, trehalose, glycerol, total protein and total amino acids in diapause nymphs of Laodelphax striatellus at different instars differed from those of non-diapause nymphs. The contents of glycogen and glycerol in diapause nymphs were not significantly different from those in non-diapause nymphs, but the contents of total sugar, trehalose and amino acids were significantly higher than that in non-diapause nymphs. The content of trehalose in diapause nymphs was 95.5 μg / mg, which is 1.4 times higher than that of non-diapause nymphs. The total protein content of diapause nymphs at each age was significantly higher than that of non-diapause nymphs. The diapause nymphs had the highest total protein content of 0.68 g / L, 1.3 times more than non-diapause nymphs. Studies have shown that individuals of the SBPH individuals belong to the trehalose-accumulating type, and through the diapause system to regulate physiological and biochemical habits to enhance resistance, thereby surviving the adverse environment.