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目的探讨培养脊髓神经元中溶酶体的影响因素。方法原代分离细胞培养大鼠脊髓神经元,培养细胞荧光染色,酶组织化学染色。结果培养的脊髓神经元随培养天数的增加,神经元的突起延长,且突起间逐渐相互连接。神经元中溶酶体经酸性磷酸酶(ACPase)染色后可见散在分布于神经元的胞体及突起内,细胞核ACPase反应阴性。经吞噬荧光黄(luciferyellow,LY)证实,培养的脊髓神经元在施加佛波醇肉豆蔻酸乙酯(PMA)后可见脊髓神经元中胞体及突起均有荧光,且荧光呈网络状分布,胞核无荧光。说明PMA有利于胞体及突起的生长。结论培养脊髓神经元中,溶酶体存在于胞体及突起中,PMA影响脊髓神经元的形态以及溶酶体在神经元的分布
Objective To investigate the influencing factors of lysosomal formation in spinal cord neurons. Methods Primary cultured rat spinal cord neurons were cultured in vitro. Fluorescent staining and enzymatic histochemical staining of cultured cells were performed. Results The number of neurons in cultured spinal cord neurons increased with the number of days of culture, and the neurites became longer and the neurites gradually connected with each other. Lysosomal neurons in the acid phosphatase (ACPase) staining showed scattered in the neurons of the somatic cells and protrusions, negative ACPase nuclear. After lucifeye yellow (LY) phagocytosis, the neurons of spinal cord were found to have fluorescence in the cytoplasm and protrusions in the spinal cord neurons after the application of phorbol myristate (PMA) Nuclear non-fluorescent. PMA is conducive to the growth of cell bodies and protrusions. Conclusion In culture of spinal cord neurons, lysosomes are present in cell bodies and protrusions. PMA affects the morphology of spinal cord neurons and the distribution of lysosomes in neurons