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目的 提供一种适合于临床应用的缓慢低温冷冻和快速解冻复苏的贮存人卵母细胞的方法。 方法 接受试管婴儿 (IVF)或卵子胞浆内单精子注射 (ICSI)治疗的 6例患者剩余的卵母细胞共 33个 ,通过程序化缓慢低温冷冻方法贮存于含有蔗糖的 1,2丙二醇中。 6例因不同原因需要受卵者的子宫内膜用激素替代治疗 (HRT)法调整或采用自然周期 ,当受卵者子宫内膜处于合适时机时 ,复苏冻存的卵母细胞 6次共 33个 ,共 2 8个成熟卵子通过ICSI受精 ,记录卵母细胞复苏率、复苏卵ICSI后的受精率 ,及随后胚胎发育情况 ,选择质量好的胚胎进行移植 ,胚胎移植后 2周检查受孕情况 ,孕 4周B超检查。 结果 当用高浓度的蔗糖冻存液保存时 ,慢冻快融法得到了很高的存活率。复苏后的卵子存活率 97% (32 / 33) ,复苏卵经ICSI后的受精率 82 % (2 3/ 2 8) ,受精胚胎分裂率 91% (2 1/ 2 3) ,6个胚胎移植周期共移植胚胎 2 1个。 4例获临床妊娠 ,目前 1例孕 17周 ,1例孕 12周 ,1例孕 7周 ,均为单胎妊娠 ,状态良好 ;另外 1例妊娠 9周后自然流产。 结论 人卵母细胞慢冻快融低温贮藏方法能够提高人卵母细胞冻存后的存活率。该法操作简便、费用低、复苏率高 ,较好地解决了现有人卵母细胞的技术冻存效果不理想、复苏率低的问题 ,为进一步人卵?
OBJECTIVE: To provide a method for slow cryo-freezing and rapid thawing of stored human oocytes that are suitable for clinical use. Methods Thirty-three remaining oocytes from 6 patients undergoing IVF or intracytoplasmic sperm injection (ICSI) were stored in 1,2-propanediol containing sucrose by programmed slow cryogenic freezing. In 6 cases, endometrium of ovariectomized patients was adjusted by hormone replacement therapy (HRT) or natural cycle was used for different reasons. When frozen endometrium was in the right time, cryopreserved oocytes were resuscitated 6 times A total of 28 mature eggs were fertilized by ICSI. The recovery rate of oocytes, the fertilization rate after ICSI recovery, and the subsequent embryo development were selected. Good quality embryos were selected for transplantation. The pregnancy was checked 2 weeks after embryo transfer, Pregnancy 4 weeks B-ultrasound. Results When frozen in a high concentration of sucrose solution, the rapid freezing and thawing method yielded a high survival rate. The survival rate of the recovered eggs was 97% (32/33), that of the fertilized eggs was 82% (23/28) after ICSI, and that of the fertilized embryos was 91% (21/2) Cycle a total of 21 embryos transplanted. 4 cases of clinical pregnancy, the current 1 case of 17 weeks of pregnancy, 1 case of 12 weeks of pregnancy, 1 case of pregnancy 7 weeks, are singleton pregnancies, in good condition; the other 1 case of spontaneous abortion after 9 weeks of pregnancy. Conclusion Cryopreservation of human oocytes by cryopreservation can enhance the survival rate of human oocytes after cryopreservation. The method has the advantages of simple operation, low cost and high recovery rate, which solves the problem that the existing technology of human oocyte cryopreservation is not satisfactory and the recovery rate is low, and is further human egg?