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1、染色法。①用靛兰胭脂红染色。靛兰胭脂红(兰靛)是一种苯胺染料,具有容易透过种子的死细胞使其染上颜色,但不能透过活的细胞的一特性,根据种胚着色的情况,可以区别出有生命力的,还是无生命力的种子(栎类种子不适于使用这个方法。因栎类种胚含有大量单宁、死亡细胞也不易着色。②碘——碘化钾染色。根据松、云彬、落叶松树木的种胚,在开始发芽时生成淀粉这一特性,把种子置于发芽
1, staining method. ① with indigo carmine staining. Indigo carmine (Indigo indigo) is an aniline dye with dead cells that easily penetrate the seed to color it, but not through the characteristics of a living cell. According to embryo coloring, vitality can be distinguished , Or non-viable seeds (oak seeds are not suitable for this method because oak embryos contain a large number of tannins, dead cells are not easy to stain.) ② iodine - potassium iodide staining. According to Song, Yunbin, larch trees Embryo, the characteristic starch is formed at the beginning of germination, placing seeds in germination